Comparison of High-resolution Melting Curve Analysis with Specific Target Gene Sequencing to Identify the Most Common Species of Aspergillus and Fusarium

Background: Currently, it appears that new molecular-based methods could substitute microscopic and culture assessment for the first-line detection of microorganisms isolated from clinical specimens. However, will remain "continual strategy" until this technology is attuned to identifying all fungi can be biological Objectives: The present study aimed validate a high-resolution melting (HRM) technique identify filamentous fungi. Moreover, was attempted compare results with those target gene’s polymerase chain reaction (PCR) sequencing. Methods: A total 54 specimens bronchoalveolar lavage (BAL), nail, ear discharge, blood culture, cornea were collected patients suspected fungal infection. All Fusarium spp. Aspergillus recognized based on Tef-α beta-tubulin region sequencing, as well PCR-HRM analysis. Results: sequence analysis revealed most frequent solani followed by F. oxysporum (n = 3), caucasicum coeruleum falciforme 1), proliferatum brevicatenulatum globosom verticillioides 1). Based sequences, flavus 10), A. fumigatus 7), niger 2), terreus orezea 1) identified in study. Furthermore, dataset 33 isolates belonging 24), 21) included 11), Conclusions: sequencing method has time-consuming costly nature, there exists conformity between Tef-α/beta-tubulin regions PCR-HRM. reliable approach laboratory some closely related show no curve algorithm differences